Boldenone ELISA Test Kit Plasma, Serum, Milk, Fish, Shrimp, Beef, Chicken, Pork And Urine.Up

The unique features of the kit are:

1.Rapid (10-40min), high recovery (≥80%), and cost-effective extraction methods .

2.High sensitivity (0.2 ng/g or ppb) and low detection limit (0.2 ng/g or ppb) .

3.A quick ELISA assay (less than 1.5 hours regardless of number of samples).

4.High reproducibility.

Meat(Fish,Shrimp,Beef,Chicken,Pork)

• Homogenize a reasonable amount of sample with a suitable mixer.
• Weigh out 2g of the homogenize sample into a suitable vial and mix thoroughly with 8mL of acetonitrile, vigorously vortex for 2min.

Note:The sample should be well- scattered!

• Keep the vial standing for 1 min and then transfer 1mL of the acetonitrile into a new vial. Dry the sample absolutely in a 60-70℃ water bath by blowing nitrogen gas.
• Add 0.25mL of Sample Diluent and vortex for 15s;
• Use 50μL of the sample solution per well for the assay.

Note: Dilution Factor :1

Milk

• High-fat milk should be defatted by centrifuging;
• Pipette 2mL of the milk sample and add 0.5mL of methanol, vortex for 15s
• Use 50μL per well for the assay.

Note:Dilution Factor:1.25

Urine

• Centrifuge 1 mL of the urine sample at 4,000 x g for 5 minutes.
• Pipette 0.5mL of the urine supernatant and mix with 0.4mL of distilled water and 0.1mL of methanol, vortex for 15s;
• Use 50μL per well for the assay.

Note: Dilution Factor:2

Plasma/Serum

• Pipette 1mL of the homogenize sample into a suitable vial and mix with 4mL of acetonitrile, vigorously vortex for 2min.
• Keep the vial standing for 1min and then transfer 2mL of the acetonitrile into a new vial. Dry the sample absolutely in a 60-70℃ water bath by blowing nitrogen gas.
• Add 0.2 mL of distilled water and vortex for 15s;
• Use 50μL of the sample solution per well for the assay.

Note: Dilution Factor: 0.5